I haven't read all of Ultimate Spider-Man, and I haven't read any of the Lizard story in Ultimate team up, but I figured I'd start this thread to discuss things about the movie that seemed to be more Ultimate than 616.
I only have a few, I'm sure you guys will notice a lot more.
-Flash plays basketball.. Peter can dunk
-Peter accidentally breaks a lot of things when first discovering his powers.
-Richard and Mary Parker are scientists, not spies
-Uncle Ben's great responsibility speech seemed similar to the one in Ultimate
-Uncle Ben's death happens after Peter has a temper tantrum and runs out on Ben and May.. Asking rhetorically where his father is
I know there were a few more but I wasn't taking notes.